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Image Search Results
Journal: Nature medicine
Article Title: Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain.
doi: 10.1038/s41591-023-02644-6
Figure Lengend Snippet: Fig. 5 | CSF-CCM promotes proliferation and NSC response in hiPS cell- derived astrocytes by LGALS3BP. a, Schematic timeline of experimental paradigm. b, Representative micrographs showing immunostainings for the astrocyte marker S100B and FGFR3 in hiAstros. c, Disease-specific CSF effects on EdU-incorporation in hiAstros cell cultures. White arrowheads indicate examples of EdU+ hiAstros. d, The percentage of EdU+ hiAstros in different culture conditions. e,f, Representative images of immunostaining for EdU, GFAP/S100B and GAL3 of hiAstros in control (e) or CSF-containing cultures (f). Filled white arrowheads indicate GAL3+EdU+ cells; empty white arrowheads depict Gal3+EdU− astrocytes. g, Representative micrographs showing reduced hiAstros proliferation after exposure either to LGALS3BP-function blocking antibody MDP1959 alone or in combination with CSF-CCM. h,i, Phase-contrast images of primary neurospheres generated from hiAstros in control or CSF-
Article Snippet: CSF-CCM or CSF-ICM (100 μl ml−1 medium), MDP1959 (40 μl ml−1 medium) and/or
Techniques: Derivative Assay, Marker, Immunostaining, Control, Blocking Assay, Generated